Supplementary Movie 2: 48 hpf embryos injected with control (left panel) or Egfl7 antisense (middle and right panel) oligos were recorded with a video camera. Posterior trunks are shown here. Completeknockdown was achieved in some embryos resulting in the absence of circulation (the embryo shown in the middle panel is an example, and is the same embryo shown in the right panel of movie 1). Partial loss of function was achieved in a subset of embryos injected with lower dose of the antisense oligo. These embryos showed a weaker phenotype (embryo in the right panel is an example). In the embryo shown in the right panel, a segment of the two major trunk vessels failed to form lumen, thus resulting in premature returning of circulation anterior to the affected vessels. play movie ...